pgl2-sv40 vector Search Results


pgl2-sv40 vector  (Promega)
90
Promega pgl2-sv40 vector
( A ) Basal activity of reference alleles of RE candidates in HL1 (light grey bars) and COS7 cells (dark grey bars). Luciferase activity for each RE candidate is normalized to the activity of the empty <t>pGL2-SV40</t> vector. ( B ) Luciferase activity of reference alleles of RE candidates upon stimulation with Smad/Gata- (SG4) and Tcf+LiCl- (Wnt) factors in COS7 cells. Luciferase activity of each RE candidate is normalized to the activity of each RE candidate upon co-transfection with pcDNA (control). Transfections were performed in duplicates and replicated twice. Error bars represent standard deviation. *: p<0.05.
Pgl2 Sv40 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl2-sv40+vector/pgl3+basic/pmc07365664-234-11-13
Average 90 stars, based on 1 article reviews
pgl2-sv40 vector - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
vector pgl2 empty control sv40 luc  (Addgene inc)
93
Addgene inc vector pgl2 empty control sv40 luc
( A ) Basal activity of reference alleles of RE candidates in HL1 (light grey bars) and COS7 cells (dark grey bars). Luciferase activity for each RE candidate is normalized to the activity of the empty <t>pGL2-SV40</t> vector. ( B ) Luciferase activity of reference alleles of RE candidates upon stimulation with Smad/Gata- (SG4) and Tcf+LiCl- (Wnt) factors in COS7 cells. Luciferase activity of each RE candidate is normalized to the activity of each RE candidate upon co-transfection with pcDNA (control). Transfections were performed in duplicates and replicated twice. Error bars represent standard deviation. *: p<0.05.
Vector Pgl2 Empty Control Sv40 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl2-sv40+vector/pGL2-+Empty+control+-SV40-Luc+(Plasmid+%2326280)/pmc04991457-120-44-51
Average 93 stars, based on 1 article reviews
vector pgl2 empty control sv40 luc - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
plasmid pxl2622  (Promega)
90
Promega plasmid pxl2622
( A ) Basal activity of reference alleles of RE candidates in HL1 (light grey bars) and COS7 cells (dark grey bars). Luciferase activity for each RE candidate is normalized to the activity of the empty <t>pGL2-SV40</t> vector. ( B ) Luciferase activity of reference alleles of RE candidates upon stimulation with Smad/Gata- (SG4) and Tcf+LiCl- (Wnt) factors in COS7 cells. Luciferase activity of each RE candidate is normalized to the activity of each RE candidate upon co-transfection with pcDNA (control). Transfections were performed in duplicates and replicated twice. Error bars represent standard deviation. *: p<0.05.
Plasmid Pxl2622, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl2-sv40+vector/plasmid+pxl2622/us07364894-143-7-15
Average 90 stars, based on 1 article reviews
plasmid pxl2622 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
sv40- luc pgl2-control vector plasmid dna  (Promega)
90
Promega sv40- luc pgl2-control vector plasmid dna
<t>DNA</t> end-joining efficiency is decreased in DES vs. VEH MSCs. Luciferase gene reporter assay detected lesser DNA end-joining activity in DES vs. VEH MSCs following co-transfection with linearized HindIIIpGL2-control <t>luc</t> and CMV-β-gal plasmids. Means of combined experimental and biological replicates were compared using a two-tailed Student unpaired t-test. Lines represent the mean normalized end-joining activity ± SD. *P < 0.0001
Sv40 Luc Pgl2 Control Vector Plasmid Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl2-sv40+vector/plasmid+pgl2+gal4+5/pmc06203880-130-4-15
Average 90 stars, based on 1 article reviews
sv40- luc pgl2-control vector plasmid dna - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Basal activity of reference alleles of RE candidates in HL1 (light grey bars) and COS7 cells (dark grey bars). Luciferase activity for each RE candidate is normalized to the activity of the empty pGL2-SV40 vector. ( B ) Luciferase activity of reference alleles of RE candidates upon stimulation with Smad/Gata- (SG4) and Tcf+LiCl- (Wnt) factors in COS7 cells. Luciferase activity of each RE candidate is normalized to the activity of each RE candidate upon co-transfection with pcDNA (control). Transfections were performed in duplicates and replicated twice. Error bars represent standard deviation. *: p<0.05.

Journal: eLife

Article Title: Trait-associated noncoding variant regions affect TBX3 regulation and cardiac conduction

doi: 10.7554/eLife.56697

Figure Lengend Snippet: ( A ) Basal activity of reference alleles of RE candidates in HL1 (light grey bars) and COS7 cells (dark grey bars). Luciferase activity for each RE candidate is normalized to the activity of the empty pGL2-SV40 vector. ( B ) Luciferase activity of reference alleles of RE candidates upon stimulation with Smad/Gata- (SG4) and Tcf+LiCl- (Wnt) factors in COS7 cells. Luciferase activity of each RE candidate is normalized to the activity of each RE candidate upon co-transfection with pcDNA (control). Transfections were performed in duplicates and replicated twice. Error bars represent standard deviation. *: p<0.05.

Article Snippet: Amplified sequences were ligated in the XcmI site of a modified pGL2-SV40 vector (Promega) using T4 DNA Ligase (ThermoFisher Scientific; 15224–090).

Techniques: Activity Assay, Luciferase, Plasmid Preparation, Cotransfection, Transfection, Standard Deviation

( A ) Overview of the murine Tbx3 locus with ATAC_AVJ, EMERGE and STARR_SG4 data. Fragments selected for luciferase assay, selected either on STARR-seq activity or EMERGE/ATAC peaks are depicted below. ( B ) Correlation between luciferase reporter activity (fold change of reporter activity over activity of empty pGL2-SV40 vector; threshold FC >2) and STARR-seq (log2FC of SG4 over pcDNA control; threshold log2FC >0.58 (FC >1.5)) of selected fragments.

Journal: eLife

Article Title: Trait-associated noncoding variant regions affect TBX3 regulation and cardiac conduction

doi: 10.7554/eLife.56697

Figure Lengend Snippet: ( A ) Overview of the murine Tbx3 locus with ATAC_AVJ, EMERGE and STARR_SG4 data. Fragments selected for luciferase assay, selected either on STARR-seq activity or EMERGE/ATAC peaks are depicted below. ( B ) Correlation between luciferase reporter activity (fold change of reporter activity over activity of empty pGL2-SV40 vector; threshold FC >2) and STARR-seq (log2FC of SG4 over pcDNA control; threshold log2FC >0.58 (FC >1.5)) of selected fragments.

Article Snippet: Amplified sequences were ligated in the XcmI site of a modified pGL2-SV40 vector (Promega) using T4 DNA Ligase (ThermoFisher Scientific; 15224–090).

Techniques: Luciferase, Activity Assay, Plasmid Preparation

DNA end-joining efficiency is decreased in DES vs. VEH MSCs. Luciferase gene reporter assay detected lesser DNA end-joining activity in DES vs. VEH MSCs following co-transfection with linearized HindIIIpGL2-control luc and CMV-β-gal plasmids. Means of combined experimental and biological replicates were compared using a two-tailed Student unpaired t-test. Lines represent the mean normalized end-joining activity ± SD. *P < 0.0001

Journal: Biology of Reproduction

Article Title: Endocrine disruptor exposure during development increases incidence of uterine fibroids by altering DNA repair in myometrial stem cells †

doi: 10.1093/biolre/ioy097

Figure Lengend Snippet: DNA end-joining efficiency is decreased in DES vs. VEH MSCs. Luciferase gene reporter assay detected lesser DNA end-joining activity in DES vs. VEH MSCs following co-transfection with linearized HindIIIpGL2-control luc and CMV-β-gal plasmids. Means of combined experimental and biological replicates were compared using a two-tailed Student unpaired t-test. Lines represent the mean normalized end-joining activity ± SD. *P < 0.0001

Article Snippet: Plasmid DNA preparation Briefly, SV40- luc pGL2-control vector plasmid DNA ( Supplemental Figure S1A , Promega, Madison, WI) was used to transform DH5α Escherichia coli cells for plasmid amplification and was then isolated using PureYield Plasmid Midiprep System (Promega).

Techniques: Luciferase, Reporter Assay, Activity Assay, Cotransfection, Two Tailed Test